○喜多山 篤1 寺坂 知恵1 野本 佳寿恵1 西出 浩世2 新井 理3 小原 雄治3 餅井 真4 上野 直人1
基生研・形態形成1，基生研・計算機室2，遺伝研3，姫工大 ・院理・生命科学4
In order to examine global gene expression profile and gene function during the early developmental processes of Xenopus laevis, we have constructed three normalized cDNA libraries from different developmental stages of embryos (early gastula, neurula, and tailbud). To date, more than 100,000 expression sequence tags (ESTs) have been collected from these libraries. A set of 50,945 of 3'- ESTs was assembled into 19,151 of clusters as unique transcripts. Putative transcripts were functionally annotated using the Gene Ontology classification system. Many prominent genes that seem to play a key role during the early developmental processes, like as growth factors, ligands and receptors of signal pathways, etc. are listed. With the selected non-redundant clone set, we have started full-length cDNA sequencing and whole mount in situ hybridization. We also present microarray experiments combined with embryological techniques.
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